Section: New Results

Metabolic reconstruction of Klebsiella pneumoniae str. Kp13

Klebsiella pneumoniae str. Kp13 is a multidrug resistant pathogen involved in nosocomial outbreaks in Brazil. The objectives of this study still underway are two-fold: (1) to perform a graph-based metabolic reconstruction of the small-molecules network of strain Kp13 and (2) from the reconstructed network evaluate what makes this pathogen so successful in colonising its human host. Manual annotation of the network was performed and a choke-point analysis was carried out, which yielded interesting targets such as L-rhamnose biosynthesis enzymes that may be related to the virulence of this bacterium. The MetAnnot platform within MetExplore was used for the manual curation of the network and graph export/import. A paper is in preparation. This work is being done in collaboration with A. T. Vasconcelos and M. Nicolás.